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Nicholas Isaac Smith

Principal InvestigatorNicholas Isaac Smith

Associate Professor 06-6879-4945 nsmithat_markap.eng.osaka-u.ac.jp
Education history 2002 Ph.D. (Engineering/Applied Physics) Osaka University
1997 B.Sc., honours (Physics, 1st class Hons) University of Sydney
1995 B.Sc. (Physics/Pure Mathematics) University of Sydney
Research and career history 2007 - Designated Lecturer, Photonics Center, Osaka University.
2003 - 2007 Assistant Professor, Dept Frontier Biosciences, Osaka University.
2002 - 2003 JST Post-doctoral fellowship at Osaka University.
Research field Developing label-free tools for molecular imaging and analysis of the immune response

Journal papers:

  1. A. J. Hobro, N. Pavillon, K. Fujita, M. Ozkan, C. Coban and N. I. Smith, "Label-free Raman imaging of the macrophage response to the malaria pigment hemozoin", Analyst (2015) (in press).
  2. N. Pavillon and N. I. Smith, "Maximizing throughput in label-free microspectroscopy with hybrid Raman imaging", J. Biomed. Opt. 20(1), pp. 016007-1-016007-10 (2015).
  3. N. I. Smith, K. Mochizuki, H. Niioka, S. Ichikawa, N. Pavillon, A. J. Hobro, J. Ando, K. Fujita and Y. Kumagai, "Laser-targeted photofabrication of gold nanoparticles inside cells", Nat. Commun. 5(5144), pp. 1-9 (2014).
  4. M. Yamanaka, N. I. Smith and K. Fujita, "Introduction to super-resolution microscopy", Microscopy Microscopy (Tokyo) 63(3), pp. 177-192 (2014).
  5. L.-d. Chiu, A. Palonpon, N. I. Smith, S. Kawano, M. Sodeoka and K. Fujita, "Dual-polarization Raman spectral imaging to extract overlapping molecular fingerprints of living cells", J. Biophotonics (2014) (in press).
  6. K.-C. Huang, K. Bando, J. Ando, N. I. Smith, K. Fujita and S. Kawata, "3D SERS (surface enhanced Raman scattering) imaging of intracellular pathways", Methods 68(2), pp. 348-353 (2014).
  7. Y. Yonemaru, M. Yamanaka, N. I. Smith, S. Kawata and K. Fujita, "Saturated Excitation Microscopy with Optimized Excitation Modulation", Chem. Phys. Chem. 15(4), pp. 743-749 (2014).
  8. D. Pissuwan, A. J. Hobro, N. Pavillon and N. I. Smith, "Distribution of label free cationic polymer-coated gold nanorods in live macrophage cells reveals formation groups of intracellular SERS signals of probe nanoparticles", RSC Advances 4(11), pp. 5536-5541 (2014).
  9. N. Pavillon, K. Fujita and N. I. Smith, "Multimodal Label-free Microscopy", J. Innov. Opt. Health Sci. 7(5), pp. 1330009-1-22 (2014).
  10. M. Yamanaka, Y. Yonemaru, S. Kawano, K. Uegaki, N. I. Smith, S. Kawata and K. Fujita, "Saturated excitation microscopy for sub-diffraction-limited imaging of cell clusters", J. Biomed. Opt. 18(12), pp. 126002-1-126002-7 (2013).
  11. N. Pavillon, A. J. Hobro and N. I. Smith, "Cell Optical Density and Molecular Composition Revealed by Simultaneous Multimodal Label-Free Imaging", Biophys. J. 105(5), pp. 1123-1132 (2013).
  12. M. Yamanaka, K. Saito, N. I. Smith, S. Kawata, T. Nagai and K. Fujita, "Saturated excitation (SAX) of fluorescent proteins for sub-diffraction-limited imaging of living cells in three dimensions", Interface Focus 3(5), pp. 20130007 (2013)
  13. N. Pavillon, K. Bando, K. Fujita and N. I. Smith, "Feature-based recognition of Surface-enhanced Raman spectra for biological targets", J. Biophotonics 6(8), pp. 587-597 (2013).
  14. A. J. Hobro, D. M. Standley, S. Ahmad and N. I. Smith, "Deconstructing RNA: optical measurement of composition and structure", Phys. Chem. Chem. Phys. 15(31), pp. 13199-13208 (2013).
  15. A. J. Hobro, A. Konishi, C. Coban and N. I. Smith, "Raman spectroscopic analysis of malaria disease progression via blood and plasma samples", Analyst 138(14), pp. 3927-3933 (2013).
  16. D. Pissuwan, Y. Kumagai and N. Smith, "Effect of surface-modified gold nanorods on inflammatory cytokine response in macrophage cells", Part. Part. Syst. Charact. 30(5), pp. 427-433 (2013).
  17. Y. Kumamoto, A. Taguchi, N.I. Smith, and S. Kawata, "Deep ultraviolet resonant Raman imaging of a cell," J. Biomed. Opt. 17(7), pp. 076001-1-076001-4 (2012)
  18. M. Okada, N. I. Smith, A. F. Palonpon, H. Endo, S. Kawata, M. Sodeoka, K. Fujita, "Label-free Raman observation of cytochrome c dynamics during apoptosis," Proceedings of the National Academy of Sciences USA, Vol.109, No.1, pp. 28-32 (2012)
  19. J. Ando, K. Fujita, N. I. Smith and S. Kawata, "Dynamic SERS imaging of cellular transport pathways with endocytosed gold nanoparticles," Nano Letters, Vol. 11, No. 12, pp 5344-5348 (2011)
  20. Y. Kumamoto, A. Taguchi, N.I. Smith, and S. Kawata, "Deep UV resonant Raman spectroscopy for photodamage characterization in cells," Biomed. Opt. Express, 2, 927-936 (2011)
  21. M. Yamanaka, Y-. K. Tzeng, S. Kawano, N. I. Smith, S. Kawata, H-. C. Chang, and K. Fujita, "SAX microscopy with fluorescent nanodiamond probes for high-resolution fluorescence imaging," Biomed. Opt. Express, Vol. 2, Issue 7, pp. 1946-1954 (2011) doi:10.1364/BOE.2.001946
  22. M. Honda, Y. Saito, N. I. Smith, K. Fujita, and S. Kawata, "Nanoscale heating of laser irradiated single gold nanoparticles in liquid," Opt. Express, Vol. 19, Issue 13, pp. 12375-12383 (2011) doi:10.1364/OE.19.012375
  23. S. Kawano, N. I. Smith, M. Yamanaka, S. Kawata and K. Fujita, "Determination of the expanded optical transfer function in saturated excitation imaging and high harmonic demodulation," Appl. Phys. Express, Vol.4, 042401 (2011)
  24. R. J. Milewski, Y. Kumagai, K. Fujita, D.M. Standley,and N.I. Smith "Automated processing of label-free Raman microscope images of macrophage cells with standardized regression for high-throughput analysis" Immunome Research 2010, 6:11doi:10.1186/1745-7580-6-11
  25. M.-L. Zheng, K. Fujita, W.-Q. Chen, N.I. Smith, X.-M. Duan, and S. Kawata "Comparison of Staining Selectivity for Subcellular Structures by Carbazole- Based Cyanine Probes in Nonlinear Optical Microscopy" ChemBioChem 2010, 11, 1 - 4 DOI: 10.1002/cbic.201000593
  26. N.I. Smith "A light to move the heart" Nature Photonics, Vol 4, September 2010, 587-589
  27. K. Fujita, S. Ishitobi, K. Hamada, N. I. Smith, A. Taguchi, Y. Inouye, and S. Kawata, "Time-resolved observation of surface-enhanced Raman scattering from gold nanoparticles during transport through a living cell," J. Biomed. Opt. Vol. 14, 024038 (2009).
  28. J. Ando, N. I. Smith, K. Fujita, and S. Kawata, " Photogeneration of membrane potential hyperpolarization and depolarization in non-excitable cells," European Biophysics Journal European Biophysics Journal, 38, 255-262 (2009).
  29. K. Fujita and N. I. Smith, "Label-free molecular imaging of living cells," Molecules and Cells, 26,. 530-535 (2008)
  30. J. Ando, G. Bautista, N. Smith, K. Fujita, and V. Daria, "Optical trapping and surgery of living yeast cells using a single laser," Review of Scientific Instruments 79, 103705 (2008).
  31. M. Yamanaka, S. Kawano, K. Fujita, N. I. Smith, and S. Kawata, "Beyond the diffraction limit biological imaging by saturated excitation (SAX) microscopy," Journal of Biomedical Optics. 13, 050507 (2008).
  32. H. Niioka, N. I. Smith, K. Fujita, Y. Inouye, and S. Kawata, "Femtosecond laser nano-ablation in fixed and non-fixed cultured cells", Optics Express, 16, 14476-14495 (2008)
  33. N. I. Smith, Y. Kumamoto, S. Iwanaga, J. Ando, K. Fujita, and S. Kawata, "A femtosecond laser pacemaker for heart muscle cells," Optics Express, 16, 8604-8616, (2008)- Also selected for Virtual Journal of Biomedical Optics. (Vol. 3, Iss. 7)
  34. K. Hamada, K. Fujita, N. Smith, M. Kobayashi, Y. Inouye, and S. Kawata, "Raman microscopy for dynamic molecular imaging of living cells," Journal of Biomedical Optics, 13, 044027 (2008).
  35. Y. Saito, M. Kobayashi, D. Hiraga, K. Fujita, S. Kawano, N. Smith, Y. Inouye, and S. Kawata, "Z-polarization sensitive detection in micro Raman spectroscopy by radially polarized incident light," Journal of Raman Spectroscopy 39,1643 - 1648 (2008).
  36. S. Iwanaga, N.I. Smith, K. Fujita, S. Kawata, "Slow Ca2+ wave stimulation using low repetition rate femtosecond pulsed irradiation", Optics Express, 14, 717-725, (2006). - Also selected for Virtual Journal of Biomedical Optics. (Vol. 1, Iss. 2)
  37. S. Iwanaga, T. Kaneko, K. Fujita, N.I. Smith, O. Nakamura, T. Takamatsu, and S. Kawata, "Location-dependent Photogeneration of calcium waves in HeLa cells," Cell Biochemistry and Biophysics, 45, 167-176 (2006)
  38. N. I. Smith, S. Iwanaga, T. Beppu, K. Fujita, O. Nakamura, and S. Kawata, "Photostimulation of two types of Ca2+ waves in rat pheochromocytoma PC12 cells by ultrashort pulsed near-infrared laser irradiation," Laser Physics Letters, 3, 154-161 (2006).
  39. S. Iwanaga, N.I. Smith, K. Fujita, S. Kawata, and O. Nakamura, "Single pulse cell stimulation with a near-infrared picosecond laser", Appl. Phys. Lett., 87, 243901 (2005).
  40. M. R. Arnison, K. G. Larkin, C. J. R. Sheppard, N. I. Smith, and C. J. Cogswell, "Linear phase imaging using differential interference contrast microscopy", Journal of Microscopy., 214, 7-12 (2004).
  41. P. A. Robinson, I. H. Cairns, and N. I. Smith, "Unified Theory of Monochromatic and Broadband Modulational and Decay Instabilities of Langmuir Waves", Physics of Plasmas , 9, 4149-4159, (2002).
  42. N. Smith, K. Fujita, T. Kaneko, K. Kato, O. Nakamura, T. Takamatsu, and S. Kawata, "Generation of calcium waves in living cells by pulsed laser-induced photodisruption," Appl. Phys. Lett., 79, 8, 1208-1210 (2001).
  43. N. Smith, K. Fujita, O. Nakamura, and S. Kawata, "Subsurface microprocessing of collagen in 3D by ultrashort laser pulses," Appl. Phys. Lett., 78, 999-1001 (2001).
  44. M. R. Arnison, C. J. Cogswell, N. I. Smith, P. W. Fekete, and K. G. Larkin, "Using the Hilbert transform for 3D visualization of differential interference contrast microscope images", Journal of Microscopy, 199, 79-84, (2000).
  45. I. H. Cairns, P. A. Robinson, and N. I. Smith, "Arguments against modulational instabilities of Langmuir waves in Earth's foreshock", Journal of Geophysical Research, 103 (A1), 287-299, (1998).
  46. 43.  C. J. Cogswell, N. I. Smith, K. G. Larkin, and P. Hariharan, "DIC microscopy made quantitative using a geometric phase shifter", Cell Vision (Now Applied Immunohistochemistry and Molecular Morphology), 4, 243-244, (1997)

Book chapters:

  1. Y. Kumamoto, N.I. Smith, K. Fujita, J. Ando, S. Kawata, "Optical techniques for future pacemaker technology" Book chapter in press (Intech publishing)
  2. N. I. Smith, S. Kawano, M. Yamanaka, and K. Fujita, "Nonlinear fluorescence imaging by saturated excitation" in Nanoscopy Multidimensional Optical Fluorescence Microscopy, pp. 2-1 ~ 2-16 (A. Diaspro Ed., Chapman and Hall/CRC press, April 2010).
  3. N. Smith, S. Iwanaga, H. Niioka, K. Fujita, and S. Kawata, "Subcellular effects of femtosecond laser irradiation" in Nano Biophotonics - (H. Masuhara, S. Kawata, and F. Tokunaga, Ed. Elsevier B.V., Amsterdam, 2007)
  4. S. Kawata, O. Nakamura, T. Kaneko, M. Hashimoto, K. Goto, N.I. Smith, T. Sugiura, I. Fujimasa, and H. Matsumoto, "Biological Imaging and Sensing from Basic Techniques to Clinical Application" in Biological Imaging and Sensing - (T Furukawa Ed. Springer, 2004)
  5. K. Fujita, N. Smith, and O. Nakamura, "Nonlinear optical imaging and stimulation of living cells," in Nanophotonics -Intengrating photochemistry, Optics and Nano/Bio Materials Studies - (H. Masuhara and S. Kawata Ed. Elsevier B.V., Amsterdam, 2004).