Kazuya Kikuchi's group (Chemical Imaging Techniques) and Masaru Ishii's group (Immunology and Cell Biology) designed and developed a functional small molecular probe (SMP)
with an active-targeting moiety, higher photostability, and fluorescence switch, and imaged target cell activity by injecting the SMP into living mice.
The combination of the rationally designed SMP with a fluorescent protein as a reporter of cell localization enabled quantitation of osteoclast activity and time-lapse
imaging of its in vivo function associated with changes in cell deformation and membrane fluctuations. Real-time imaging revealed heterogenic behaviors of osteoclasts
in vivo and provided insights into the mechanism of bone resorption.
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Contact:
Kazuya Kikuchi
Chemical Imaging Techniques Science
Immunology Frontier Research Center (WPI-IFReC), Osaka University
Masaru Ishii
Immunology and Cell Biology
Immunology Frontier Research Center (WPI-IFReC), Osaka University