News & Topics
Isolation of tissue-resident vascular endothelial stem cells from mouse liver (Takakura G, in Nat. Protocols)
Endothelial cells (ECs) are fundamental components of the blood vessels that comprise the vascular system; facilitate blood flow; and regulate permeability, angiogenesis, inflammatory responses and homeostatic tissue maintenance. The group of Nobuyuki Takakura (Signal Transduction, IFReC/RIMD, Osaka University) describes an easy and reproducible protocol for isolation of different types of ECs and CD157+ vascular-resident endothelial stem cells (VESCs) by mechano-enzymatic tissue digestion followed by fluorescence-activated cell sorting.
Article (External Link)