Human Immunology
（Single Cell Genomics)

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Human Immunology

Immunology Researchers and Laboratories Rely Heavily on Single-Cell Analysis
Our advantages: Cutting-edge immunology research + Proximity to Osaka University Hospital + IFReC Human Immunology Lab
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Our project: Constructing the world’s largest, most advanced, and highest quality “IFReC original” single immune cell database
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Our goals: Exploring new realms of immunology and contributing to medical care



State-of-the-art equipment for single cell analysis of human samples

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Single Cell Analysis

Brief introduction of Single Cell Analysis

Advances in single-cell analysis technology in basic research and clinical research have enabled us to understand genetic information at the single-cell level, which was impossible until now from the population-level views of cells.

Advantage of Single Cell Analysis

It becomes possible to identify a cell population exhibiting a rare and hidden characteristic which can be identified as a therapeutic target, thereby leading to possible drug development.

How to contribute to Human Immunology

Standards in single-cell analysis have not yet been set and many issues remain, such as data collection and analysis methods. As an early adopter in Japan of single-cell analysis for clinical use, we have the knowledge, experience, and infrastructure to handle all processes, from cell harvesting to data analysis.

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Principal Investigator

Daisuke Okuzaki Associate Professor

Research field:Molecular biology-related, Cell biology-related
+81-6-6879-4935(IFRec Lab) / +81-6-6879-8323 (Biken lab)
dokuzakibiken.osaka-u.ac.jp
Education history
1995.03 BSc, Faculty of Engineering, Okayama University, Japan
1997.03 MEng, Graduate School of Engineering, Okayama University, Japan
2001.03 PhD, Osaka University, Japan
Research and career history
2002.1 Assistant, Research Institute for Microbial Diseases, Osaka University
2004.4 DNA Chip Development Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University
2007.4 Assistant Professor, DNA Chip Development Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University
2017.4 Assistant Professor, NGS Core, Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University
2019.11 Specially Appointed Associate Professor, Immunology Frontier Research Center (IFReC), Osaka University

Members

Shota Nakamura(concur.) 　Associate Professor
nshotagen-info.osaka-u.ac.jp
Daisuke Motooka(concur.)　Assistant Professor
daisukemgen-info.osaka-u.ac.jp
Masakazu Ishikawa 　Researcher
mishikawaifrec.osaka-u.ac.jp
Ayako Takuwa 　Researcher
takuwaifrec.osaka-u.ac.jp
Yu-Chen Liu 　Researcher
yuchenliuifrec.osaka-u.ac.jp
 
 

Publications

1: Al Kadi M, Jung N, Ito S, Kameoka S, Hishida T, Motooka D, Nakamura S, Iida T, Okuzaki D. UNAGI: an automated pipeline for nanopore full-length cDNA sequencing uncovers novel transcripts and isoforms in yeast. Funct Integr Genomics. 2020 Jan 18. doi: 10.1007/s10142-020-00732-1. [Epub ahead of print] PubMed PMID: 31955296.
2: Okuzaki D, Yamauchi T, Mitani F, Miyata M, Ninomiya Y, Watanabe R, Akamatsu H, Oneyama C. c-Src promotes tumor progression through downregulation of microRNA-129-1-3p. Cancer Sci. 2020 Feb;111(2):418-428. doi: 10.1111/cas.14269. Epub 2020 Jan 17. PubMed PMID: 31799727; PubMed Central PMCID: PMC7004518.
3: Hasegawa T, Kikuta J, Sudo T, Matsuura Y, Matsui T, Simmons S, Ebina K, Hirao M, Okuzaki D, Yoshida Y, Hirao A, Kalinichenko VV, Yamaoka K, Takeuchi T, Ishii M. Identification of a novel arthritis-associated osteoclast precursor macrophage regulated by FoxM1. Nat Immunol. 2019 Dec;20(12):1631-1643. doi: 10.1038/s41590-019-0526-7. Epub 2019 Nov 18. PubMed PMID: 31740799.
4: Okuzaki D, Ota K, Takatsuki SI, Akiyoshi Y, Naoi K, Yabuta N, Saji T, Nojima H. FCN1 (M-ficolin), which directly associates with immunoglobulin G1, is a molecular target of intravenous immunoglobulin therapy for Kawasaki disease. Sci Rep. 2017 Sep 12;7(1):11334. doi: 10.1038/s41598-017-11108-0. PubMed PMID: 28900133; PubMed Central PMCID: PMC5595863.
5: Okuzaki D, Yoshizaki K, Tanaka T, Hirano T, Fukushima K, Washio T, Nojima H. Microarray and whole-exome sequencing analysis of familial Behçet's disease patients. Sci Rep. 2016 Jan 20;6:19456. doi: 10.1038/srep19456. PubMed PMID: 26785681; PubMed Central PMCID: PMC4726226.
6: Nagi-Miura N, Okuzaki D, Torigata K, Sakurai MA, Ito A, Ohno N, Nojima H. CAWS administration increases the expression of interferon γ and complement factors that lead to severe vasculitis in DBA/2 mice. BMC Immunol. 2013 Sep 24;14:44. doi: 10.1186/1471-2172-14-44. PubMed PMID: 24063402; PubMed Central PMCID: PMC3876726.