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HOME > News & Topics > Research > FY 2008 > GPHR is a novel anion channel critical for acidification and functions of the Golgi apparatus. (Associate Prof. Maeda in Nature Cell Biology)

GPHR is a novel anion channel critical for acidification and functions of the Golgi apparatus. (Associate Prof. Maeda in Nature Cell Biology)

The organelles within secretory and endocytotic pathways in mammalian cells have acidified lumens, and regulation of their acidic pH is critical for the trafficking, processing and glycosylation of cargo proteins and lipids, as well as the morphological integrity of the organelles. How organelle lumen acidification is regulated, and how luminal pH elevation disturbs these fundamental cellular processes, is largely unknown.

The authors describe a novel molecule involved in Golgi acidification.

First, mutant cells defective in Golgi acidification were established that exhibited delayed protein transport, impaired glycosylation and Golgi disorganization. Using expression cloning, a novel Golgi-resident multi-transmembrane protein, named Golgi pH regulator (GPHR), was identified as being responsible for the mutant cells. After reconstitution in planar lipid bilayers, GPHR exhibited a voltage-dependent anion-channel activity that may function in counter ion conductance.

Thus, GPHR modulates Golgi functions through regulation of acidification.


Article


Contact:

Yusuke Maeda
Laboratory of Immunoglycobiology,
WPI Immunology Frontier Research Center, Osaka University,
3-1 Yamada-oka, Suita, Osaka 565-0871, Japan

Tel: +81-6-6879-8329 Fax:+81-6-6875-5233
E-mail: ymaeda@biken.osaka-u.ac.jp

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