Endothelial cells (ECs) are fundamental components of the blood vessels that comprise the vascular system; facilitate blood flow; and regulate permeability, angiogenesis, inflammatory responses and homeostatic tissue maintenance. The group of Nobuyuki Takakura (Signal Transduction, IFReC/RIMD, Osaka University) describes an easy and reproducible protocol for isolation of different types of ECs and CD157+ vascular-resident endothelial stem cells (VESCs) by mechano-enzymatic tissue digestion followed by fluorescence-activated cell sorting.​ ​

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Nobuyuki Takakura（Signal Transduction）

+81-6-6879-8316
ntakakubiken.osaka-u.ac.jp

Immunology Frontier Research Center, Osaka University (WPI-IFReC)