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Isolation of tissue-resident vascular endothelial stem cells from mouse liver (Takakura G, in Nat. Protocols)​

Endothelial cells (ECs) are fundamental components of the blood vessels that comprise the vascular system; facilitate blood flow; and regulate permeability, angiogenesis, inflammatory responses and homeostatic tissue maintenance. The group of Nobuyuki Takakura (Signal Transduction, IFReC/RIMD, Osaka University) describes an easy and reproducible protocol for isolation of different types of ECs and CD157+ vascular-resident endothelial stem cells (VESCs) by mechano-enzymatic tissue digestion followed by fluorescence-activated cell sorting.​ ​

Isolation of tissue-resident vascular endothelial stem cells from mouse liver

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Contact:
Nobuyuki Takakura

Nobuyuki Takakura(Signal Transduction)


Tel+81-6-6879-8316
Mailntakakubiken.osaka-u.ac.jp

Immunology Frontier Research Center, Osaka University (WPI-IFReC)

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